Julio C. Ruiz and colleagues from University of Texas Southwestern Medical Center report that gene trap-mediated disruption of the ubiquitously expressed murine F-box and leucine-rich repeat protein 5 (Fbxl5) gene results in a failure to sense increased cellular iron availability, accompanied by constitutive IRP2 accumulation and misexpression of IRP2 target genes. FBXL5-null mice die during embryogenesis, while mice containing a single functional Fbxl5 allele behave like their wild type littermates when fed an iron-sufficient diet. However, unlike wild type mice that manifest decreased hematocrit and hemoglobin levels when fed a low-iron diet, Fbxl5 heterozygotes maintain normal hematologic values due to increased iron absorption. These results confirm the role of FBXL5 in the in vivo maintenance of cellular and systemic iron homeostasis and reveal a privileged role for the intestine in their regulation by virtue of its unique FBXL5 iron sensitivity. The FBXL5 knockout used in this study can be obtained here.
Texas A&M Institute for Genomic Medicine (TIGM) facilitates breakthroughs in science and medicine and accelerate the pace of medical discoveries through internal research and by providing our resources, training and services to the scientific community at Texas A&M, The State of Texas, and the world.
The Texas A&M Institute for Genomic Medicine (TIGM) is an essential resource for any researcher looking to obtain knockout mice and embryonic stem (ES) cells quickly and with favorable intellectual property (IP) terms. Our resources include the world’s largest gene trap library of ES cells in the C57BL/6N mouse strain and access to the largest library of ES cells in the 129/SvEvBrd mouse strain. TIGM provides both ES cell clones and mice to the public and private international research community.
Since beginning its operation in 2006, TIGM has served as a major resource to the international scientific community. In that time, TIGM has delivered more than 800 mouse and ES cells orders to more than 270 academic and commercial institutions in over 26 countries. In addition, a total of over 7,100 individual investigators from more than 900 academic and research institutions and commercial entities representing 40 countries, have queried TIGM with information requests.These resources are being used with great success and to date there are at more than 50 peer-reviewed publications featuring TIGM mice or cells.
For the complete list of publications please visit http://www.tigm.org/publications/
The TIGM mouse knockout database currently includes links identifying embryonic stem cell clones from the C57BL/6 and 129S5/SvEvBrd gene trap libraries, as well as more than 2,500 established mouse knockout lines from the 129 line. Together, these resources cover more than 13,000 mouse genes and can be searched by gene or protein sequence, accession number, chromosome, gene ID or keyword. TIGM also offers more than 200 established cryopreserved lines in our mouse repository.
C57BL/6 Mice and ES Cell Clones
TIGM operates a gene trap library – a premier knockout mouse ES cell resource – that contains over 350,000 cell lines in the C57BL/6 mouse background. This library contains mutated ES cell clones representing more than 10,000 genes.
TIGM has access to a privately held 129S5/SvEvBrd gene trap library. This gene trap library contains more than 270,000 sequence-tagged embryonic stem cell clones in the 129/SvEvBrd mouse strain representing mutations in over 10,000 genes. The National Institutes of Health (NIH) and Wellcome Trust Sanger Institute have obtained rights to a subset of these lines (NIH Lines and Wellcome Trust Lines), allowing TIGM to make them available for distribution to the academic research community on a subsidized basis. Please note that some of the 129S5/SvEvBrd lines in the database are not available to the public as they are reserved due to commercial proprietary research.
|Download our brochure||The Texas A&M Institute for Genomic Medicine (TIGM) is a part of the Texas A&M University System as a research institute of Texas A&M AgriLife Research. TIGM utilizes advanced technologies to discover breakthroughs in science and medicine and accelerate the pace of medical discoveries. TIGM accomplishes this through internal research and collaborations with other institutions. TIGM also maintains the world’s largest library of mouse knockout embryonic stem cells and provides both ES cells and mice to academic and commercial institutions around the world.|
Publication describing our library and technology can be found here: