Transgenic Services

TIGM Transgenic Core Services and Fees

(academic and non-profit customers; for commercial fees please contact TIGM at agolovko@tigm.org)

Prices effective November 1, 2023


Pronuclear/Cytoplasmic Injection (including CRISPR/Cas9*)

$3,960

Investigator provides at least 30 ug of digested plasmid DNA, detailed map of the construct and a picture of the DNA with the desired band circled. In case of CRISPR, investigator provides uncut plasmid vector expressing sgRNA, sequences of the sgRNA and template DNA vector of oligo as needed. Includes injection of at least 125 FVB embryos. All resulting offspring will be transferred to the investigator at weaning. TIGM can also genotype the offspring for the transgene at an extra cost.

Pronuclear Injection, other strains (C57BL/6, etc.)

Basic Pronuclear Injection + Additional $928

Pronuclear Injection of BAC DNA

Basic Pronuclear Injection + Additional $928
Please contact the core to obtain protocols for preparing BAC DNA. BAC DNA needs to be submitted to the core as a pellet under 70% ethanol.

*Listed rates apply to indels and point mutations only. For more complex projects (conditional knockouts and knock-ins) please contact us at info@tigm.org to obtain a detailed quote.

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Blastocyst Injection

$2,846

Investigator provides two vials of the frozen clone. The service includes injection of at least 30 blastocysts/clone. All resulting offspring will be transferred to the investigator at weaning.

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Sperm Cryopreservation

$681

Sperm from two male mice will be cryopreserved using the protocol from the Jackson Laboratory. The cryopreservation fee includes sperm viability testing by in vitro fertilization. After the first year there is a biannual $121 liquid nitrogen storage fee.

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Sperm Cryopreservation (Jax kit)

$1,815

Sperm from two male mice will be cryopreserved using the kit from the Jackson Laboratory. The viability of the frozen sperm will be tested at the Jackson Laboratory by in vitro fertilization and stored at the Jackson Laboratory. After 3 years there is a $170 storage fee per strain per year.

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Embryo Cryopreservation

$1,980

Investigator transfers 6-8 practiced stud males, 3-6 months of age, to TIGM quarantine. The core will perform two sessions and will cryopreserve at least 200 embryos at the morula stage. Additional sessions can be performed at a cost of $880. The cryopreservation fee includes embryo viability testing. After the first year there is a biannual $121 liquid nitrogen storage fee.

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Rederivation via IVF (live mice)

$1,856

Investigator transfers 2-3 practiced stud males, 3-6 months of age, to TIGM quarantine for sperm collection. The core will isolate oocytes from at least 5 superovulated females (C57BL/6, FVB or CRD1 ICR strains; other strains could be used too, but additional charges related to animal order may apply), perform IVF and transfer the resulting embryos to recipient females. All resulting offspring will be transferred to the investigator at weaning. The remaining sperm sample can be cryopreserved and stored at TIGM. After the first year there is a biannual $121 liquid nitrogen storage fee.

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Rederivation via IVF (sperm samples prepared using Jax protocol)

$1,485

Investigator provides at least 2 straws each containing at least 40-50 ul of frozen sperm. The core will isolate oocytes from at least 5 superovulated females(C57BL/6, FVB or CRD1 ICR strains; other strains could be used too, but additional charges related to animal order may apply), perform IVF and transfer the resulting embryos to recipient females. All resulting offspring will be transferred to the investigator at weaning.

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Rederivation via IVF (sperm samples prepared using other protocols)

$4,331

Investigator provides at least 4 straws/vials each containing approximately 200 ul of frozen sperm. The core will perform two sessions and will use oocytes from 20 donors per vial of sperm. C57BL/6, FVB or CRD1 ICR strains could be used as donors; other strains could be used too, but additional charges related to animal order may apply.The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.

[More details] [Download Form]


Rederivation via Embryo Transfer

$1,980

Investigator transfers 3-6 practiced stud males, 3-6 months of age, to TIGM quarantine for mating with superovulated females. The core will perform 1-2 sessions and will transfer at least 100 embryos at the morula stage.

[More details] [Download Form]


Frozen Embryo Transfer

$557

Investigator provides at least 4 straws each containing approximately 20 embryos. 2 straws will be used for transfer. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.

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Live Embryo Transfer

$557

Investigator provides early stage embryos in M2 medium or alternatively freshly isolated uterine or oviduct tissues from timed pregnancies for the core to flush. The investigator must coordinate with the core at least a week in advance so that the core can set up recipient females at the appropriate stage. All resulting offspring will be transferred to the investigator at weaning.

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Colony maintenance

$2.37 per cage/day

Includes general husbandry, health monitoring, breeding, weaning, ear tagging, tail clipping and genotyping as needed.

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Gene Targeting via Homologous Recombination (constitutive, conditional or knock-in)

Services include:

  • Targeting vector construction and designing of the screening strategy
  • Transfection of ES cells and confirmation of targeted clones
  • ES cell blastocyst injection
  • Chimera Breeding
  • Genotyping protocol design and confirmation

Services can be requested individually or as a package. Prices may vary depending on complexity of a project. Please contact TIGM for more information.

 


Electroporation service package

$4,579

The service includes linearization/purification/ electroporation of targeting construct; isolation/expansion of drug-resistant colonies in 96-well format (at least 2 plates); preparation of cell pellet plates in 96-well format and expansion of all targeted ES cell clones as specified by the customer.

Additional services:

Identification of targeted ES cell clones by PCR $ 1,485 per plate

Expansion of targeted ES cell clones $ 326 per clone

 

[More details] [Download Form]


 

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