Transgenice Core services offered to TAMUS Faculty
TIGM had completed its move from Houston to College Station during August 2010. We now have the personnel, expertise, facilities and equipment to provide mouse transgenic core services for the Texas A&M System community. Those services are offered to our TAMUS colleagues at cost. For more information about the services TIGM can offer you please email Dr. Andrei Golovko at: agolovko@tigm.org.Form to Transfer Animals Between TAMUS facilities
Animal Transfer Form (doc) TIGM Transgenic Core Service | |
| Service and Description | Cost |
|---|---|
| Blastocyst Injection | $2,100 |
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Investigator provides two vials of the frozen clone. The service includes injection of at least 30 blastocysts/clone. All resulting offspring will be transferred to the investigator at weaning. [More details] [Download Form] | |
| Pronuclear Injection | $2,600 |
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Investigator provides at least 20 ug of plasmid DNA at a concentration of 500 ug/ml. Includes injection of at least 125 FVB embryos. All resulting offspring will be transferred to the investigator at weaning. [More details] [Download Form] | |
| Rederivation via embryo transfer | $1,100 |
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Investigator transfers 3-6 practiced stud males, 3-6 months of age, to TIGM quarantine for mating with superovulated females. The core will perform 1-2 sessions and will transfer at least 100 embryos at the morula stage. [More details] [Download Form] | |
| Rederivation via IVF (TIGM or Jax sperm samples) | $1,100 |
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Investigator provides at least 2 straws each containing at least 40-50 ul of frozen sperm. The core will isolate oocytes from at least 5 superovulated females, perform IVF and transfer the resulting embryos to recipient females. All resulting offspring will be transferred to the investigator at weaning. [More details] [Download Form] | |
| Rederivation via IVF (Other Samples) | $3,200 |
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Investigator provides at least 4 straws each containing approximately 200 ul of frozen sperm. The core will perform two sessions and will use oocytes from 20 donors per vial of sperm. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning. [More details] [Download Form] | |
| Frozen Embryo transfer | $375 |
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Investigator provides at least 4 straws each containing approximately 20 embryos. 2 straws will be used for transfer. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning. [More details] [Download Form] | |
| Live Embryo transfer | $375 |
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Investigator provides early stage embryos in M2 medium or alternatively freshly isolated uterine or oviduct tissues from timed pregnancies for the core to flush. The investigator must coordinate with the core at least a week in advance so that the core can set up recipient females at the appropriate stage. All resulting offspring will be transferred to the investigator at weaning. [More details] [Download Form] | |
| Embryo Cryopreservation | $1,400 |
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Investigator transfers 6-8 practiced stud males, 3-6 months of age, to TIGM quarantine. The core will perform two sessions and will cryopreserve at least 200 embryos at the morula stage. Additional sessions can be performed at a cost of $650. The cryopreservation fee includes embryo viability testing. After the first year there is a biannual $100 liquid nitrogen storage fee. [More details] [Download Form] | |
| Sperm Cryopreservation | $500 |
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Sperm from two male mice will be cryopreserved using the protocol from the Jackson Laboratory. The cryopreservation fee includes sperm viability testing by in vitro fertilization. After the first year there is a biannual $100 liquid nitrogen storage fee. [More details] [Download Form] | |
| Sperm Cryopreservation (Jax Kit) | $1,500 |
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Sperm from two male mice will be cryopreserved using the kit from the Jackson Laboratory. The viability of the frozen sperm will be tested at the Jackson Laboratory by in vitro fertilization and stored at the Jackson Laboratory. After 3 years there is a $170 storage fee per strain per year. [More details] [Download Form] | |
| Electroporation service package | $4,600 |
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The service includes linearization/purification/ electroporation of targeting construct; isolation/expansion of drug-resistant colonies in 96-well format (at least 2 plates); preparation of cell pellet plates in 96-well format and expansion of all targeted ES cell clones as specified by the customer. [More details] [Download Form]
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| Cryostorage (2 year fee) | $100 |
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The samples will be stored in MVE Model 815-190F liquid nitrogen vapor freezers
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