Mouse Reproductive Technology Workshop

Hosted by Texas A&M Institute for Genomic Medicine (TIGM)

College Station Texas, USA

14-18 November, 2017



Description of Course

The studies performed in the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have enhanced the mouse cryopreservation field with state-of-the-art methods for mouse cryopreservation, including embryo and sperm cryopreservation, in vitro fertilization, embryo and sperm transport, and superovulation methods.

The aim of this course is to provide hands-on training by Prof. Nakagata and his scientific team in these new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities. This is only the second time that Prof. Nakagata has conducted this course in the United States.

Hands-on topics covered in the course include:Eggs with cumulus mass
Isolating unfertilized mouse oocytes
Isolating and cold storage/shipping of mouse cauda epididymis
Isolating and cold storage of embryos
Freezing/thawing mouse sperm and IVF
Fresh mouse sperm and IVF
Cold Storage sperm and IVF
Freezing/thawing 2-cell IVF-derived mouse embryos
Vitrification of mouse oocytes and embryos
Ovary transplantation/ovary freezing
Embryo transfer techniques in mice (oviduct, uterus)
NSET training

Additional lecture topics will include:
Historic and scientific perspectives of embryo and sperm cryopreservation
Comparing current embryo and sperm cryopreservation methods
Vitrification of oocytes and their use for IVF
New US Guidelines for the use of animals in research/IACUC
Cold storage and transport of germplasm
Large archiving and distribution centers- challenges and solutions
Shipping mice, refrigerated and frozen material
CRISPR/Cas9 genome editing updates


The official language of the course will be English. All practical sessions and lectures will be held in English


CARD-Texas Workshop Schedule



Naomi Nakagata (CARD-Kumamoto University, Japan)
Toru Takeo (CARD-Kumamoto University, Japan)
Tomoko Kondo (CARD-Kumamoto University, Japan)
Yukie Haruguchi (CARD-Kumamoto University, Japan)
Yumi Takeshita (CARD-Kumamoto University, Japan)
Yuko Nakamuta (CARD-Kumamoto University, Japan)
Shuuji Tsuchiyama (CARD-Kumamoto University, Japan)
Ayumi Mukunoki (CARD-Kumamoto University, Japan)
Hidetaka Yoshimoto (CARD-Kumamoto University, Japan)
Andrei Golovko (TIGM, College Station TX, USA)
Bill Shawlot (UT-Austin, Austin TX, USA)
Benjamin Morpurgo (TIGM, College Station TX, USA)
Jan Parker-Thornburg (MD Anderson, Houston, TX, USA)
Kristy Williams (Mouse Biology Program, UC Davis, CA, USA)
Barbara Stone (ParaTechs Corp, Lexington, KY, USA)

Additional lectures by

Naomi Nakagata (CARD-Kumamoto University, Japan)
Toru Takeo (CARD-Kumamoto University, Japan)
Jorge Sztein (Barcelona, Spain)
John Stallone (Texas A&M University, College Station TX, USA)
Charles Long (Texas A&M University, College Station TX, USA)

Registration Information

Registration fee: $1500 USD ($1400 USD for ISTT members)

Number of participants: 20

To apply please register here or send a CV and a cover letter describing how the applicant and his/her laboratory will benefit by attending the workshop to Additional letters of reference may be submitted. Applications will be reviewed beginning July 1st. We expect to begin notifying applicants of their acceptance by July 15th. The workshop is open to a maximum of 20 students. Applicants not initially selected will be placed on a waiting list and will be invited based on any cancellations or unpaid registrations as of September 1st, 2017. Wait list applicants will be notified of their final status by September 8th, 2017.

The course registration fee is $1500 USD ($1400 USD for ISTT Members). This fee includes participation in the entire course, all materials and reagents, lunches for 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, at a convenient nearby hotel where all instructors and lecturers will be also lodged.

Travel Information

A block of rooms has been secured at our recommended hotel: Shuttle transportation will be arranged for course participants from this hotel to the workshop site at no additional cost. Cavalry Court is in walking distance to a variety of restaurants.

TIGM is located in College Station, Texas, USA on the campus of Texas A&M University.  It is located approximately 1.5 hours by car or shuttle from George Bush Intercontinental Airport (airport code: IAH), or just under 2 hours away from William P. Hobby Airport (airport code: HOU).  The shuttle from IAH to College Station costs approximately $45/person (one-way).

TIGM Building
[Texas A&M Institute for Genomic Medicine, College Station, TX]


Texas A&M University, College Station, TX    Downtown, Houston, TX




[Texas A&M University, College Station, TX]                                                                                                                                                   [Downtown, Houston, TX]


Contact Information

Contact: Andrei Golovko/ Stephanie King

Contact email:

Contact telephone: +1 979-845-8446

Contact fax: +1 979-458-5559

Organized By

Naomi Nakagata (CARD-Kumamoto University, Japan),

 Toru Takeo (CARD-Kumamoto University, Japan),

Benjamin Morpurgo (TIGM, USA)

Andrei Golovko (TIGM, USA)

Bill Shawlot (University of Texas at Austin, USA)

Jan Parker-Thornburg (MD Anderson Cancer Center, USA)


CARD Logo TIGM Logo Texas A&M AgriLife Research Logo University of Texas LogoMD Anderson Cancer Center Logo


Fertility of cold-stored mouse sperm is recovered by promoting acrosome reaction and hyperactivation after cholesterol efflux by methyl-beta-cyclodextrin. Yoshimoto H, Takeo T, Irie T, Nakagata N. Biol Reprod. 2017 Feb 1;96(2):446-455. doi: 10.1095/biolreprod.116.142901.

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice. Nakagawa Y, Sakuma T, Nishimichi N, Yokosaki Y, Yanaka N, Takeo T, Nakagata N, Yamamoto T. Biol Open. 2016 Aug 15;5(8):1142-8. doi: 10.1242/bio.019349.

Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains. Takeo T, Nakagata N. Theriogenology. 2016 Sep 15;86(5):1341-6. doi: 10.1016/j.theriogenology.2016.04.076. Epub 2016 May 7.

N-acetyl cysteine prolonged the developmental ability of mouse two-cell embryos against oxidative stress at refrigerated temperatures. Horikoshi Y, Takeo T, Nakagata N. Cryobiology. 2016 Jun;72(3):198-204. doi: 10.1016/j.cryobiol.2016.05.002. Epub 2016 May 7.

Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. Takeo T, Nakagata N. PLoS One. 2015 May 29;10(5):e0128330. doi: 0.1371/journal.pone.0128330. eCollection 2015.Cryobiology. 2016 Jun;72(3):198-204. doi: 10.1016/j.cryobiol.2016.05.002. Epub 2016 May 7.

Cysteine analogs with a free thiol group promote fertilization by reducing disulfide bonds in the zona pellucida of mice. Takeo T, Horikoshi Y, Nakao S, Sakoh K, Ishizuka Y, Tsutsumi A, Fukumoto K, Kondo T, Haruguchi Y, Takeshita Y, Nakamuta Y, Tsuchiyama S, Nakagata N.  Biol Reprod. 2015 Apr;92(4):90. doi: 10.1095/biolreprod.114.125443. Epub 2015 Feb 25.

Contemporary techniques for freezing mouse spermatozoa. Guan M, Bogani D, Marschall S, Raspa M, Takeo T, Nakagata N, Taft R, Fray M.  Curr Protoc Mouse Biol. 2014 Sep 3;4(3):85-104. doi: 10.1002/9780470942390.mo140065.

In vitro fertilization in mice using the MBCD-GSH protocol. Guan M, Bogani D, Marschall S, Raspa M, Takeo T, Nakagata N, Fray M.  Curr Protoc Mouse Biol. 2014 Jun 16;4(2):67-83. doi: 10.1002/9780470942390.mo140059.

Transporting mouse embryos and germplasm as frozen or unfrozen materials. Kenyon J, Guan M, Bogani D, Marschall S, Raspa M, Pickard A, Takeo T, Nakagata N, Fray M.  Curr Protoc Mouse Biol. 2014 Jun 16;4(2):47-65. doi: 10.1002/9780470942390.mo140064.

Conservation of mouse models through embryo freezing. Guan M, Bogani D, Marschall S, Raspa M, Takeo T, Nakagata N, Fray M.  Curr Protoc Mouse Biol. 2014 Dec 11;4(4):205-27. doi: 10.1002/9780470942390.mo140082.

Prolonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes. Ishizuka Y, Takeo T, Nakao S, Yoshimoto H, Hirose Y, Sakai Y, Horikoshi Y, Takeuji S, Tsuchiyama S, Nakagata N.  J Reprod Dev. 2014;60(6):454-9. doi: 10.1262/jrd.2014-045. Epub 2014 Sep 12.

Lipocalin 2 binds to membrane phosphatidylethanolamine to induce lipid raft movement in a PKA-dependent manner and modulates sperm maturation.Watanabe H, Takeo T, Tojo H, Sakoh K, Berger T, Nakagata N, Mak TW, Kondoh G.  Development. 2014 May;141(10):2157-64. doi: 10.1242/dev.105148.

Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm Nakagata, N, Takeo T, Fukomoto K, Haruguchi Y, Kondo T, Takeshita Y, Nakamuta Y, Umeno T, Tsuchiyama S.  Journal of Reproduction and Development. 2014 Apr 24;60(2):168-71

Applications of cryopreserved unfertilized mouse oocytes for in vitro fertilization.  Nakagata N, Takeo T, Fukumoto K, Kondo T, Haruguchi Y, Takeshita Y, Nakamuta Y, Matsunaga H, Tsuchiyama S, Ishizuka Y, Araki K.Cryobiology. 2013 Oct;67(2):188-92.

Reduced glutathione enhances fertility of frozen/thawed C57BL/6 mouse sperm after exposure to methyl-beta-cyclodextrin. Takeo T, Nakagata N. Biol Reprod. 2011 Nov;85(5):1066-72.

Combination medium of cryoprotective agents containing L-glutamine and methyl-{beta}-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse sperm. Takeo T, Nakagata N. Lab Anim. 2010 Apr;44(2):132-7.

Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. Takeo T, Hoshii T, Kondo Y, Toyodome H, Arima H, Yamamura K, Irie T, Nakagata N. Biol Reprod. 2008 Mar;78(3):546-51.

 Texas State Capitol, Austin, TX

[Texas State Capitol, Austin, TX]


Kumamoto Castle, Kumamoto Prefecture 860-0002, Japan
[Kumamoto Castle, Kumamoto Prefecture 860-0002, Japan]



Kyudo Logo

Charles River logo

Leica Microsystems Logo



Comments are closed.